ACTUALLY THERNZ
It was never really implied in Castlevania III that the other characters weren't aware of her gender. It was kind off meant as a suprise for the player, but there is no evidence that this was also the case in-game.
Yeah, we don't know what Trevor thought, or how long he knew she was a woman officially. But the end of her story in Judgment did confirm that she actually did enter Transylvania disguised as a man, partly because of all the witch burnings in the area. That actually cleared up some stuff for me. I had thought she had pretended to be a man the entire time she was under the service of the church and was then shocked to see she wasn't really hiding it in Judgment.
On another note, it would be cool if this game was actually a preview of the next big Iga game to come out. Maybe they're just waiting until the Lords of Shadows sequels are done before making the game. Hey, I can be optimistic, can't I?
On yet another note, I'm hoping to see some good ole classic Dracula's Curse bosses. Maybe instead of cards randomly being selected to determine who you'll fight, a coffin will randomly open, and out will come a Cyclops, pair of Mummy Men, or a Leviathon?